Bioconversion by gut microbiota of predigested mango (Mangifera indica L) ‘Ataulfo’ peel polyphenols assessed in a dynamic (TIM-2) in vitro model of the human colon

Gut microbiota bioconversion of polyphenols in predigested mango ‘Ataulfo’ peel was studied using a validated, dynamic vitro human colon model (TIM-2) with faecal microbial inoculum. Dried peels were enzymatic treatment, followed by TIM-2 fermentation (72 h). Samples taken at 0, 24, 48 and 72 h analyzed HPLC-QToF. Derivatives hydroxyphenylpropionic, hydroxyphenylacetic hydroxybenzoic acids, as well as, pyrogallol the main identified. These metabolites might derivate from flavonoid (flavanols flavonols), gallate gallotannin biotransformation. Despite high content ellagic acid peel, low amounts detected samples due to transformation into urolythins A C, mainly. Xanthone benzophenone derivatives, specific mango, remained after colonic biotransformation, contrary flavonoids, which completely disappeared. In conclusion, microbial-derived metabolites, such xanthone among others, are partially stable fermentation, thus have potential contribute bioactivity.